Part:BBa_K1132010:Design

Tp901 integrase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 28
  Illegal AgeI site found at 1486
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination.

The 180° switch permits to design a lot of regulation tools, such as logical gates that can be found here (https://parts.igem.org/Part:BBa_K1132001, https://parts.igem.org/Part:BBa_K1132002).

attB + attP + integrase → attR + attL + integrase

Source

later